Cytochrome Oxidase 2 (COX2), β-Tubulin (TUB) and Chitin Synthase Subunit 2 (CHS2) Expression in Pythium insidiosum Thai Strains

This study aimed to investigate the real-time fluctuation of temperature-sensitive gene expression in a Pythium insidiosum growing at human body temperature (37 ºC) when it can cause life-threatening disease, whereas its natural habitat is agricultural water sources with lower temperature. Genes encoding cytochrome oxidase 2 (COX2), β-tubulin (TUB), and chitin synthase subunit (CHS2) were tested for their infection (27 using RT-PCR. Sixteen strains P. insidiosum, representing 3 phylogeographic preferences, analyzed. The subculturing process 27 ºC was repeated least - cycles. results showed that 37 ºC, COX2 expressed 2.5-fold higher than (p = 0.0347) while level TUB CHS2 mRNA disclosed indistinguishable profiles. These suggested these genes suitable be used as housekeeping temperature-susceptible studies. work likely 1st examining levels Thai strain under thermal stress conditions. Follow-up studies may useful provide valuable insight into pathogenesis, diagnostic, or therapeutic targets further investigation.